Coding

Part:BBa_M36624:Design

Designed by: Jodie Sheffels, Benjamin Yeh, Ali Hoffer   Group: Stanford BIOE44 - S11   (2015-10-22)

Antifungal protein from Aspergillus giganteus (active)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This DNA sequence encodes the mature AFP protein produced by Aspergillus giganteus. The 51 amino acid peptide is regarded as the active form of the AFP protein; longer precursors, including the 94-amino-acid preproprotein that we have also documented in the Parts Registry (Part BBa_M36623), appear to be less potent in terms of antifungal activity (Meyer).

Since this part was designed for use in E. coli, the exact DNA sequence was generated by submitting the amino acid sequence to IDT Codon Optimizer (https://www.idtdna.com/CodonOpt). We opted for the "Escherichia coli K12" codon table and selected the first result..


Source

http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1990.tb19300.x/full This links to the amino acid sequence, which we then entered into IDT Codon Optimizer to find the optimal corresponding DNA sequence in E. coli.

References

1. Meyer, Vera. "A small protein that fights fungi: AFP as a new promising antifungal agent of biotechnological value." Applied microbiology and biotechnology 78.1 (2008): 17-28. <http://link.springer.com/article/10.1007/s00253-007-1291-3/fulltext.html> 2. 51 amino acid sequence: http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1990.tb19300.x/full 3. https://parts.igem.org/Part:BBa_M36624